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2 edition of Genetic analysis of a region of the Rhizobium Sym plasmid pRLIJI. found in the catalog.

Genetic analysis of a region of the Rhizobium Sym plasmid pRLIJI.

Anastassios Economou

Genetic analysis of a region of the Rhizobium Sym plasmid pRLIJI.

by Anastassios Economou

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  • 20 Currently reading

Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D), University of East Anglia, School of Biological Sciences, 1990.

ID Numbers
Open LibraryOL14592665M

Studies on Rhizobium meliloti Plasmids 39 detected whatever the endonuclease used, either giving a great number of fragments such as EcoRI, Hind or BamHI or a low number such as SmaI, KpnI or Xbal. Southern DNA/DNA hybridization experiments were performed for more pre- cise estimation of sequence by: This book provides in-depth reviews of the role of Rhizobium in agriculture and its biotechnological applications. Individual chapters explore topics such as: the occurrence and distribution of Rhizobium; phenotypic and molecular characteristics of Rhizobium; impact of Rhizobium on other microbial communities in the rhizosphere; N2-fixation ability of Rhizobium; Rhizobium and biotic stress.

In the N 2 ‐fixing bacterium Rhizobium leguminosarum, mutations in a homologue of tonB (tonB Rl) block the import of vicibactin and haem as iron sources in free‐living bacteri.   TECHNICAL FIELD. This invention relates to a symbiotic plasmid of the broad host-range Rhizobium sp. NGR and its use. In particular, this invention relates to the isolation and analysis of the complete sequence of the NGR symbiotic plasmid pNGRa, and the open reading frames (ORFs) identifiable therein as well as the proteins expressible from said ORFs.

P>Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn. Agrobacterium tumefaciens). Requirement of a Plasmid-Encoded Catalase for Survival of Rhizobium etli CFN42 in a Polyphenol- Complementation analysis revealed that the katG gene located on this plasmid, encoding the only catalase present in this bacterium, restored full resistance to testa .


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Genetic analysis of a region of the Rhizobium Sym plasmid pRLIJI by Anastassios Economou Download PDF EPUB FB2

A region of kb of the Sym(biosis) plasmid pRL1JI of Rhizobium leguminosarum, consisting of the established kb nodulation region which confers nodulation ability on Vicia hirsuta and a region of kb which appeared to be necessary for nodulation on V. sativa and Trifolium subterraneum, was subcloned as fragments of maximally kb in a newly developed IncQ Cited by: Wijffelman CA, Pees E, van Brussel AAN, Okker RJH, Lugtenberg BJJ () Genetic and functional analysis of the nodulation region of the Rhizobium leguminosarum Sym plasmid pRLlJI.

Arch Microbiol – CrossRef Google ScholarCited by: 7. Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.

J Bacteriol. Jul; (1)– [PMC free article] Friedman AM, Long SR, Brown SE, Buikema WJ, Ausubel FM. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. by: A plasmid of Mdal from Rhizobium leguminosarum RCC was found to be a Sym plasmid (pSym1) carrying genes for root nodulation and nitrogen fixation on plants of the pea vetch cross-inoculation group.

The plasmid was expressed not only in different R. leguminosarum and R. trifolii hosts, but also in Agrobacterium tumefaciens and R. meliloti, although in root nodules induced by A Cited by: Abstract. A kilobase (kb) fragment of Rhizobium trifolii Sym plasmid containing nodulation (nod) genes or the pSym plasmid of R.

trifolii cointegrated with a broad-host-range vector R (pPN1) were transferred to Lignobacter strain K17 and Pseudomonas aeruginosa strain PAO5 by by: A procedure for the genetic transformation of Thizobium meliloti by plasmid DNA is destribed.

It is an adaptation of a procedure used originally for Escherichia coli and later for Agbaterium ing on the R. meliloti isolate used as the recipient, the efficiency of transformation was in the range of 7 × 10 1 to × 10 3 per μg of plasmid pRK DNA and 10 −6 per viable by: Amplification and deletion of nod-nif region in the symbiotic plasmid of Rhizobium phaseoli Article (PDF Available) in Journal of Bacteriology (8) May with 28 Reads.

upon a Rhizobium Plasmid Origin of Replication and Its Application to Genetic Engineering of Rhizobium Strains. Doctor of Philosophy (Molecular Biology), May,pp., 6 tables, 31 illustrations, bibliography, titles. Rhizobia are Gram-negative, rod-shaped, soil bacteria with the ability to fix atmospheric nitrogen into ammonia asAuthor: Pyengsoo Jeong.

Genetic structure of the conjugation system on pRleVF39b and alignment with other type IV rhizobial plasmid conjugation systems. (A) The transfer genes (in gray) on pRleVF39b contain a.

1 Introduction. Bacterial genomes harbour extra chromosomal elements such as plasmid and transposable elements. Plasmids, in fact, constitute 25–50% of the total DNA in some bacteria including Rhizobium[].A number of analyses at the molecular level have revealed fine differences among rhizobia and led to the characterization of plasmid genotype and hence refinement of lineages [2,4].Cited by:   Molecular genetic and physiological analysis of Rhizobium-rice interactions.

Download ( MB) link to publisher version. regions of the pSymA plasmid suggested that genes involved in rice growth inhibition were located in the Kb deleted region of pSymA that is deleted from strain SmA This region has genes involved in nitrate Author: Francine Manuella Perrine.

Symbiotic bacteria known as rhizobia interact with the roots of legumes and induce the formation of nitrogen-fixing nodules. In rhizobia, essential genes for symbiosis are compartmentalized either in symbiotic plasmids or in chromosomal symbiotic islands.

To understand the structure and evolution of the symbiotic genome compartments (SGCs), it is necessary to analyze their common. Rhizobium strains nodulating legumes are considered to be particularly symbiosis-specific because of their genetic characteristics [32, 35].

Nitrogen-fixing symbiosis can be used to increase soil fertility especially for nutrient deficient soils but also to restore ecosystems disturbed or contaminated by toxic compounds such as heavy metals [26].

Rhizobia are Gram-negative, rod-shaped, soil bacteria with the ability to fix atmospheric nitrogen into ammonia as symbiont bacteroids within nodules of leguminous plant roots.

Here, resident Rhizobium plasmids were studied as possible sources of components for the construction of a cloning vector for Rhizobium : Pyengsoo Jeong. Genetic Analysis of a pH-Regulated Operon to a bp intergenic region located upstream of lpiA, the first gene in the operon.

lpiA-like genes are found in several, and Proteobacteria that interact with eukaryotic a genetic analysis of the loci encoding for these pH Cited by: 5 islands that carry the genes needed for symbiosis.

Here, we report an analysis of the 6 distribution and genetic conservation of the IS found in the genome of Rhizobium etli 7 CFN42, in a collection of 87 Rhizobium strains belonging to populations of different 8 geographical origins. We used PCR to generate presence/absence profiles of the 39 IS.

The mosaic structure of the symbiotic plasmid of The symbiotic plasmid is a circular molecule ofbase-paigenes common to other rhizobia are clustered in a region of throughout.

In some cases the mobile elements flank blocks of The plasmid contains 12 reiterated DNA families that are likely to participate in genomic rearrangements. Whether you've loved the book or not, if you give your honest and detailed thoughts then people will find new books that are right for them.

1 Toxic Interfaces of Neurones, Smoke and Genes: Proceeding of the European Society of Toxicology Meeting Held in Kuopio, June 16–19, Summary. Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn.

Agrobacterium tumefaciens).However, DNA:DNA hybridization with R. radiobacter, R. rubi, R. vitis and R. huautlense gave only 44%, 5%, 8% and 8%. Among Rhizobium species, six species include plant pathogens as a member: R. radiobacter species complex (including R. nepotum and R.

pusense), R. rhizogenes, R. vitis, R. rubi, R. larrymoorei, and R. on their pathogenic states, the members belonging to the six species can be divided into three types, namely, crown gall bacteria carrying Ti plasmid, hairy root bacteria Cited by: 1. the capacity to destabilise the corresponding plasmid in incompatibility assays, and in some cases destabilise other plasmids as well.

REFERENCES Hynes, M.F., R. Simon, and A. Pühler. () The development of plasmid-free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC Plasmid.Rhizobium sp.

NGR contains a plasmid-borne locus that hybridized strongly to region II of R. singly, NGR region II completely inhibited nodulation of Medicago sativa when conjugated into either R. meliloti nodIJ or nodQ 1 region II mutants. Further characterization showed that region II of NGR contains three putative coding open reading frames (ORFs), which are homologous.As was indicated by Beringer (8), future genetic studies of Rhizobium will be dominated by studies of plasmids.

One group of plasmids which have captured considerable interest among investigators are referred to as the symbiotic (SYM) plasmids.

One such example is the R. leguminosarum SYM plasmid, pRL1JI. This plasmid, modified by the.